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The naphthoquinone menadione is metabolized by several enzymes through one electron reduction resulting in highly reactive unstable semiquinones 77

Fujinaka Y, Takane K, Yamashita H, Vasavada RC (2007) Lactogens Promote Beta Cell Survival through JAK2/STAT5 Activation and Bcl-XL Upregulation

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Geroprotective and Anticarcinogenic Activity Lifetime Murine Studies Long-term studies in mice demonstrated that DSIP administration over a lifetime decreased total spontaneous tumour incidence 2.6-fold compared to untreated controls, slowed age-related switching-off of oestrous function, decreased the frequency of chromosome aberrations in bone marrow cells by 22.6%, and increased maximum lifespan by 24.1% compared to control groups a multi-parameter geroprotective profile that is among the most striking reported for any short regulatory peptide, and that has generated sustained interest in the DSIP analogue research field

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the protein concentration was determined photometrically ( 280 nm = 26,930 M 1 cm 1 ) and the protein was diluted into 20 l aliquots