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After 28 days, discard any remaining contents and open a new vial

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That's well below toxic thresholds, but chronic administration over 6+ months hasn't been studied in controlled human trials

Understanding Vitamin B12 and Its Role in Nerve Health Vitamin B12 is key to nerve health

These include methionine (an essential amino acid involved in metabolism), inositol (a sugar alcohol involved in fat removal), and choline (an amine involved in the metabolism of fat)

[3] [9] Those with neurological symptoms may require more frequent dosing initially (alternate days until improvement) followed by two-monthly maintenance