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Significant increases of the main saturated FAs, palmitic (C16:0) and stearic (C18:0) acid, were observed while PUFA fatty acids such as arachidonic (20:4, AA), andrenic (22:4), eicosapentaenoic acid (20:5, EPA), docasapentaenoic acid (22:5, DPA), and doxosahexaenoic acid (22:6, DHA) were decreased

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Each sample was amplified in triplicate, and the comparative Ct (Ct) value method was used for relative quantification

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