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After cross-linking treatment, the cells were washed with DEPC-treated PBS and collected in ice-cold, RNase-free IP150 lysis buffer (prepared using DEPC-treated water, consisting of 50 mM HEPES pH 7.5, 150 mM NaCl, 10% glycerol (G8190, Solarbio, Beijing, China), 0.5% Triton X-100 (T8200, Solarbio, Beijing, China), 1 mM EDTA, 1 mM DTT, 1 l RNase inhibitor, and protease inhibitor)

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A standard curve should be generated for each set of samples assayed

S., Woldeyohannes, H

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