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In 2026, as our understanding of complex biological systems deepens, the demand for exact, reproducible experimental conditions has never been higher

After an additional 4 days ( t = 96 h), cells were collected by trypsinization, pelleted, snap-frozen and stored at 80 C

FIGURE 4 (B) Mechanism of cellular ferroptosis in diminished ovarian reserve: In the ovary, iron accumulation may increase due to aging, oxidative stress, or pathological conditions (Sze et al., 2022

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In mammalian cells, ER stress leads to an integrated stress response involving the related transmembrane kinases Ire1, Ire1 and PKR-like ER kinase (PERK), and the transmembrane activating transcription factor 6 (ATF6) (Zhang & Kaufman, 2004)