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supernatants were quantified by BCA assay (Thermo, 23227) and denatured at 95C for 5 min in 5 SDS loading buffer containing dithiothreitol (DTT, 100 mM)

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This guide covers the biochemistry that answers "is HCG a peptide," what the hormone actually does in the body, its legitimate uses in fertility and men's health, its role alongside testosterone therapy, and why the weight-loss version is a myth the FDA has already knocked down

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