Site II (orange) was similar for both states but presented subtle differences, with an additional cholesterol pocket involving the Trp in extracellular loop 2 (ECL2) being more pronounced in the inactive versus the active state
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Be careful not to touch or contaminate the needle
Acknowledgments This research was supported by a Grant-in-Aid for Exploratory Research (grant number 16K20081) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan, Tokyo, Japan