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After harvesting, lysis was performed in buffer containing 50 mM Tris pH 7.6, 150 mM NaCl, and 0.5% NP-40, on ice via tissue grinding, and then proteasomes were isolated using M2 FLAG Affinity Gel (A2220

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Crucially, IBD management has shifted from merely controlling symptoms to halting disease progression through intestinal reparative therapy. In this context, true repair is strictly defined by objective endpoints such as endoscopic healing and histologic healing, rather than subjective clinical remission