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Whole blood samples for FA analysis were kept on ice, frozen at 20 C (within 30 min) for 24 h, then stored at 80 C until analysis

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However, which signaling pathway produces the unwanted side effects (such as respiratory suppression and constipation) is currently controversial: one view is that it is caused by -arrestin signal transduction, and the other view suggests that the respiratory depression by opioids is caused due to G protein-gated inwardly rectifying potassium (GIRK) channel signaling

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doi: 10.3389/fphar.2025.1438318