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Turn the vial upside down with the needle still in the vial

Front Endocrinol (Lausanne) 3:34916

"I've known Sly before I worked with him, and then he called me about this," Grillo noted

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Before and after incubation with the corresponding secondary antibodies (goat anti-rabbit IgG Alexa Fluor 488 (polyclonal, used at 1:400, Thermo Fisher Scientific, Cat# A-11008, RRID:AB_143165), goat anti-guinea pig IgG Alexa Fluor 568 (polyclonal, used at 1:400, Thermo Fisher Scientific, Cat# A-11075, RRID:AB_141954)) for 45 min, each coverslip was washed three times with PBS and finally mounted on glass slides in Immumount (Thermo Fisher Scientific)

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