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The proteins were separated by SDS-polyacrylamide gel electrophoresis and transferred to PVDF membranes before incubation with an antibody against -KA-lysil adduct (neuroketals antibody, C-17, 1:400) and anti-DAT (MAB369, 1:1000)

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Niemann-Pick type C proteins promote microautophagy by expanding raft-like membrane domains in the yeast vacuole

Darker skin tends to produce thicker lesions and may respond better to treatments specifically made for those symptoms