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This standardized culture condition ensured the appropriate growth and maintenance of the cell lines throughout the experimental procedures

Specifically, GLP-1RAs downregulate activation of VCAM1, the intracellular adhesion molecule-1 (ICAM-1) and plasminogen activator inhibitor type 1 (PAI-1) in endothelial cells [57]

These changes appear to enhance neurodegeneration with clinical manifestation of acute encephalopathy marked by lethargy, hypertonia, poor feeding, and ketoacidosis 47

These findings provide evidence that the generation of an intracellular source of cAMP is involved in GLP-1mediated cardioprotection

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