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At the end of the study the mice were euthanized, and the livers were collected and quickly washed twice in ice-cold phosphate-buffered saline (PBS)

Unlike many peptide ligands that possess well-characterized receptor targets and signaling pathways, DSIP remains associated with an incompletely defined mechanism of action

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It keeps it ready to neutralize free radicals

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Exploiting structure similarity in refinement: automated NCS and target-structure restraints in BUSTER