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Sucrose preference test as a measure of anhedonic behavior in a chronic unpredictable mild stress model of depression: outstanding issues

Day 3-4: Cochlea were fixed in 4% PFA overnight then immunohistochemically processed with the following: anti-MyoVIIa (1:500), anti-Neurofilament (1:500), Phalloidin (1:250), and DAPI (1:1000)

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RNA interference We transfected shRNA into extracted epithethial cells using Lipofectamine 3000 (#L3000008, Thermo Fisher, USA) according to the manufacturers instruction

Dosage and Route: Sublingual tablets are dissolved under the tongue roughly 2-3 hours prior to bedtime, on an empty stomach, depending on individual patient response

Yue J, Lpez JM