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Specifically, BDNF was necessary for differentiation but not the proliferation of newborn neurons, as the only difference between BDNF knockdown and controls was the number of cells expressing both proliferation marker (BrdU) and immature neuronal marker (DCX), whereas the total number of proliferating cells, either 24 h (Figure 5b) or 7 days (Figure 5c) after BrdU injections, was unchanged

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either a general-purpose medium such as Hanks Balanced Salt Solution (HBSS), which may be supplemented with Ca2+ if required, or a customized medium: Geys Balanced Salt Solution (GBSS) is routinely used for hepatic cells

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Oncologist 2013