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Samples were resuspended in 2% acetonitrile (ACN), 0.05%TFA and peptide concentrations were estimated with 205 nm absorbance readings (Nanodrop, Thermo Fischer)

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The LIP enhances intracellular iron flux through the interactions of IRPs with iron-responsive elements (IREs) in the promoter of iron metabolism-related genes, thereby upregulating the transcription and ultimately the protein expression of iron-absorbing proteins, such as ferritin, TfR1, FPN, and DMT1

Next, the correlation between the expression levels of GPX4 and miRNA-324-3p in clinical specimens was examined

Most patients have reached their primary treatment objectives by this point