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The column was then washed with 20 CV of DPBS/0.05% (v/v) sodium azide, followed by elution with 9 CV of 2 M MgCl 2 (Sigma-Aldrich)/20 mM Tris-HCl (Sigma-Aldrich) buffer (pH 7.4) at a flow rate of 2 mL/min

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This difference in status is essential to understand, and it is the purpose of the reminder below

Long term virological suppression has been shown to significantly reduce the risk of progression to cirrhosis, hepatocellular carcinoma (HCC), and liver related mortality [37, 38]