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Design: The cell-free system was designed as depicted in figure 2

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Circadian sleep wake-rhythm disorders evaluation The origin of a circadian rhythm disorder can be related either to an intrinsic abnormality in the circadian system itself (misalignment of the intrinsic circadian timing with the desired sleep schedule) or to external factors, as when individuals must be awake at times that are not synchronized with their intrinsic rhythms

2012 Aug 31;4(8):67