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Acknowledgments We thank Ping He for plasmid constructs, and Andrew Palmer, Rafael Loureiro and members of the Shippen lab for helpful discussions

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Nutr Res 46:2230 Wang QP, Browman D, Herzog H, Neely GG (2018) Non-nutritive sweeteners possess a bacteriostatic effect and alter gut microbiota in mice

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In this assay, the 30 min incubation duration was chosen to be optimal because of the inherently cytotoxic nature of CuCl 2 and Cu(OAc) 2 towards HaCaT keratinocytes, as they were observed to cause substantial cell death at a duration longer than 30 mins