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This in vitro alteration of tight junction permeability correlated with the mechanism of how PPI induced increase in tight junction permeability in vivo as PPI administration did not have a significant impact in mice lacking the myosin light chain kinase gene in the experimental colitis models (Nighot et al

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The WNK1 gene is located on chromosome 12p13.33 and is composed of 31 exons that generate four alternatively spliced mRNAs with the 2382 amino acid isoform 1 being the most common

5, 10, and 15 mg are all documented maintenance doses

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