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Stage III (72-96 hours) = Maximal stage of hepatotoxicity = Hepatic failure Clinical features Recurrence of systemic symptoms (anorexia, nausea, vomiting, malaise) Emerging signs & symptoms of hepatic failure : Encephalopathy, jaundice, coagulopathy, hypoglycemia, lactic acidosis, shock

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Stereotaxic injection The mice were administered intraperitoneal (i.p.) injections of pentobarbital sodium at a dosage of 20 mg/kg, and were then carefully positioned and adjusted on a stereotactic device (RWD Life Technologies Co., Ltd., China)