Oxidation of GSH to GSSG GSH provides the electrons needed for the enzymeindependent chemical reduction of radicals as well as for the GPxcatalyzed reduction of peroxides (Figure 2)
doi: 10.2147/jir.S493001 37 LongD.MaoC.HuangY.XuY.ZhuY
Arch Gynecol Obstet (2013) 288(1):1919
Jiao et al
Phase 3 Trial of (177)Lu-Dotatate for Midgut Neuroendocrine Tumors
Immunoblot analysis For detection of Gclc (Santa Cruz Biotechnology sc-390811), Pdha-1 (Abcam ab168379), DRP1 (Abcam ab184247), eIF4E (Abcam ab33766), p-eIF4E (Abcam ab76256), 4E-BP1 (Cell signaling 9452), p-4E-BP1 (Cell signaling 2855), DRP1 (Abcam ab184247), mTOR (Cell Signaling 2972), p-mTOR (Ser2448) (Cell Signaling 2971), p70 S6 (Cell Signaling 9202), p-p70 S6 (Thr421/Ser424) (Cell Signaling 9204), 15 10 6 cells were lysed with lysis buffer (CST 9803S) and protein/phosphatases inhibitors as per manufacturers protocol and blotted as described 44