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These cell lysates in SDC buffer (4% SDC in 100 mM Tris-HCl pH8.5) were also used for immunoblotting

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The GH locus, a 66 kb region of DNA, is located on chromosome 17q22-q24 and consists of 5 homologous genes, which appear to have been duplicated from an ancestral GH-like gene (Table 1) (273,274)

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