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Cyclically demethylated and methylated CGs of the estrogen-responsive pS2 gene are inside the CG-poor region (Metivier et al., 2008)

For subcutaneous administration, 250-500 mcg per day represents the standard range, with some protocols using 300 mcg as a target dose

This is mediated through hypothalamic ghrelin circuits and is a pharmacological action, not a side effect per se though it is often undesired in GH optimization contexts

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Mercer TR, Munro T, Mattick JS