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MOTS-c activates AMPK by disrupting the folate cycle, specifically by inhibiting AICAR transformylase, an enzyme in the de novo purine synthesis pathway

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Last Updated: November 2023

After one hour, coverslips were washed in blocking buffer, and further labeled with Alexa Fluor 488-conjugated secondary antibodies (Invitrogen, Carlsbad, CA) for one hour at RT as above

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