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W.OkunoH.BitoH.et al (2018)

Frequent temperature fluctuationscaused by opening and closing the fridge doorcan accelerate the breakdown of the peptide

Live cells were then washed with phosphate-buffered saline 1, incubated for 30 min with 5 M JC-1 (37 C, 5% CO 2 ), washed with PBS 1, trypsinized, and recovered in a final volume of 300 L of PBS

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Although the definition of carnitine insufficiency in liver cirrhosis patients remains unclear, the symptoms of carnitine deficiency are thought to be muscle cramps, general fatigue, hepatic encephalopathy, and hyperammonemia in liver cirrhosis patients