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Van Geldermalsen et al., 2016)

Activation of GHS-R1a by these ligands has been associated with differential intracellular calcium responses and variable engagement of downstream effector pathways in cell-based studies of mammalian research models

The diluted genomic DNA was added to a 10 l reaction mix containing 0.2 mg/ml BSA, 4 mM DTT, 0.10% Tween, 0.1 mM dTTP, 1X phi29 buffer, and 15 U phi 29 DNA polymerase

Grebe M, Eisele HJ, Weissmann N, et al

After mixing, 95 L of the quenched reaction were injected into an ACQUITY UPLC M-Class System with HDX Technology (Waters) 96