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48 h after transfection, cells were washed twice with pre-warmed PBS and fixed with 4% paraformaldehyde (PFA) for 1015 min

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GHK-Cu is noted to cause more injection site reactions than many other peptides

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For immunohistochemistry, detection was performed using a biotinylated anti-rabbit IgG secondary antibody and streptavidin-Horseradish peroxidase (HRP), followed by colorimetric detection using DAB