Proteomic identification and characterization of hepatic glyoxalase 1 dysregulation in non-alcoholic fatty liver disease
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Lentivirus preparation Lentiviruses were prepared as described previously ( AAV preparation AAV-293 cells (Agilent Technologies) were transfected with AAV vectors (pAAV-LStopL-GFP-shRNAmirs) and helper vectors (pAd helper vector and pAAV2/5 packaging vector) following a standard CaCl 2 method with some modifications ( density gradient ultracentrifugation and concentrated using Amicon Ultra-100 K filter unit (Millipore)
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Le rapport Firmicutes/Bacteriodetes dans le microbiote intestinal est dailleurs nettement plus lev chez les individus produisant des niveaux levs de TMAO que chez les faibles producteurs de TMAO, confortant ainsi lide selon laquelle la corrlation entre TMAO et lathrosclrose serait indirecte et probablement le rsultat dune dysbiose intestinale
