Determination of gene expression and serum levels of MnSOD and GPX1 in colorectal cancer
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Gibco), 64 mg/L ascorbic acid 2-phosphate, 14 g/L sodium selenite, 543 mg/L NaHCO 3 , 20 mg/L insulin, and 10.7 mg/L human recombinant transferrin (all from Sigma-Aldrich)), freshly supplemented with 100 ng/mL human basic fibroblast growth factor (hbFGF) and 2 ng/mL human transforming growth factor 1 (hTGFb1) (both from PeproTech)), supplemented with 10 mM Y-27632 dihydrochloride (ROCK-Inhibitor (RI)
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Funding This work was supported by the Deanship of Scientific Research, Vice Presidency for Graduate Studies and Scientific Research, King Faisal University, Saudi Arabia [Grant No