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The reduction solution reduces all the oxidized glutathione (GSSG) to each two molecules of GSH, resulting in the measurement of total GSH
Thats because glutathione, while a powerful antioxidant, is also inherently fragile
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We estimated the percent mediation of the change in eGFR at week 104, potentially attributable to change in weight, by assessing how much the treatment effect on eGFR was altered by including the weight change in the regression model adjusted for baseline eGFR and baseline weight
The hydrophobic binding site of GLP-1R ECD is occupied by hydrophobic residues of both GLP-1 and Fab 3F52
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