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Description

Cells were labelled with SNAP-Surface-649 (0.5 M, from New England Biolabs, Hitchin, UK) for 30 min at 37C

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Serum IGF-I measurement Serum IGF-I was measured centrally at qLAB (Livingston, Edinburgh, UK) using the DCP chemiluminescent immunoassay (Immulite 2000, Siemens Healthcare Diagnostics , Norwood, MA, USA)

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