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The sample size was determined pragmatically based on participant availability, and no formal power calculation was performed

Investigators studying related biological processes can use these compounds within established research frameworks while complying with applicable regulations

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Under the therapeutic concentrations, approximately 60%90% of APAP is metabolized in the liver by glucuronidation and sulfation, with a small part (approximately 5%15%) being metabolized by the cytochrome P450 pathway (CYP450) ( via two pathways to form the toxic intermediate N-acetyl-p-benzoquinone imine (NAPQI) and the non-toxic catechol metabolite 3-hydroxy-APAP (3-OH-APAP) (Figure 1)