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Following incubation at 60C overnight in 10 mM sodium citrate buffer (pH 6.0) for antigen unmasking, BLOXALL blocking solution was added to inactivate endogenous peroxidase (10 min at room temperature (RT))

23 The experts also suggested spreading information about diabetes by using social media to open up more access for general population

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Timeline matters: Power outage 12 hours: Discard