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Membranes were blocked during 1 h at room temperature in 1 PBS containing 0.1% Igepal and 5% milk and then incubated overnight at 4C with the following primary antibodies: anti-CBS mouse monoclonal antibody (ab12476, Abcam, 1:1,000) and anti--tubulin mouse monoclonal antibody (T6793, Sigma-Aldrich, 1:10,000)