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The column was then washed with 10 CV of 25 mM imidazole (Sigma-Aldrich) in DPBS (pH 7.2), followed by elution with 10 CV of 250 mM imidazole in DPBS (pH 7.3)

Additionally, patients taking GLP-1 receptor agonists are recommended to have monitoring for depression and suicidal thoughts

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Conclusion: A staged, individualized treatment plan, combined with lifestyle and nutritional optimization, ensures sustainable aesthetic outcomes for patients experiencing GLP-1 RAassociated facial changes