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Sample preparation for UHPLC-MS/MS The methanol (1.2 mL) containing the IS solution (10 g/mL, GSH- 13 C 2 , 15 N for negative ion mode (Neg)) was added to the cell-cultured plate and maintained for 2 min

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Thereafter, blood samples were obtained through the cardiac puncture into EDTA coated vials and serum samples were collected for biochemical analysis as per our previously published protocol 33

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