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Saturation mutagenesis at seven key extracellular domain sites identified positions whose substitution selectively decoupled these two signaling axes, with computational analysis of the resulting sequence-function data revealing correlations between extracellular domain residue chemistry and downstream signaling outcome (Xie et al., 2025)

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MCF7, A549, and HT-1080 cells were cultured in RPMI 1640 medium from WelGENE supplemented with 10% FBS and 1% penicillin-streptomycin