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Mistake 1: Injecting too fast Pushing the plunger rapidly forces a bolus of peptide solution into a small area of subcutaneous tissue

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7A and B, internal infusion of 10 mol/L U73122 or 60 mol/L D609 for 6 min did not change GABA currents of GCs (control), then addition of exendin-4 for 6 min still suppressed the GABA currents (73.28% 5.09% of control, P 0.05)