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RNA integrity was assessed using the RNA Nano 6000 Assay Kit and Bioanalyzer 2100 system (Agilent) to ensure high-quality RNA

All individuals provided oral informed consent to participate in the study

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There was a non-significant trend to upregulation of VSIR , encoding inhibitory checkpoint V-domain Ig suppressor of T cell activation (VISTA), in IGF-treated DU145 and 22Rv1 cells and no detectable Vsir in Myc-CaP (Supplementary Figure S4A-B)

Effect of seasonality on testicular blood flow as determined by color Doppler ultrasonography and hormonal profiles in Shiba goats

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