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The antibodies FITC mouse anti-human IFN- (cat#561057, BD Biosciences Pharmingen, CA, US), PE mouse anti-human IL-4 (cat#562046, BD Biosciences Pharmingen, CA, US), mouse anti-human IL-8 (cat#340509,BD Biosciences, Becton Dickinson Company, CA, US), PE mouse anti-human IL-17 (cat#560438, BD Biosciences Pharmingen, CA, US) and FITC mouse anti-human TNF- (cat#562082, BD Biosciences Pharmingen, CA, US) diluted in permeabilization buffer were added and incubated on ice for 30 min