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doi: 10.1111/bcpt.12360 497 HeLSuZWangS
For the CCL1, mAb1,isotype control or CCL18 binding assays, serially diluted hCCL1 (R&D systems), mAb1 (produced in-house), isotype control antibody (produced in-house) or CCL18 (R&D systems) were serially diluted in HHBS buffer in a clear 384-well plate
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The GST tag, derived from the Schistosoma japonicum parasite, facilitates the immobilization of the fusion protein on a glutathione-coated affinity column, enabling efficient protein purification and interaction studies