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For studies with a calorie-restricted and body-weight-loss-matched control group, HFHS diet (58 kcal% fat, D12331i, Research Diets) was weighed and provided as one separate pellet per mouse at the time of injection

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After the lysis of the cells with RIPA lysis buffer, the cellular protein levels were measured using a bicinchoninic acid assay (Beyotime)