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A laboratory scientist will answer your question

Yes prolonged caloric suppression without microbial repair can reduce metabolic flexibility and lower baseline energy expenditure

Mice were non-randomly assigned to treatment groups while ensuring that initial body weights were balanced across groups to minimize variability

UHPLC,ultra high performance liquid chromatography

In the Look AHEAD study, which examined the effect of intensive lifestyle intervention in patients with obesity and type 2 diabetes, the composite endpoint of adverse cardiovascular events and mortality was reduced by approximately 20% over a median follow-up period of 10.2 years, only in the group of patients that achieved a weight loss of 10% or more from their initial body weight (about 40% of the intervention arm) [7, 8]

doi:10.1016/J.AJO.2004.10.022 6