This process often creates free radicals as a byproduct, which can be more reactive than the original toxin
(2S,2'S)-5,5'-(2R,2'R)-3,3'-disulfanediylbis(1-(carboxymethylamino)-1-oxopropane-3,2-diyl)bis(azanediyl)bis(2-amino-5-oxopentanoic acid)
GPX5 is described in the epididymis as a secreted protein that contains cysteine (Cys) in the active center instead of Sec, GPX5 is the first discovered Cys-based superfamily member, also known as epididymal GPX (EGPX), which has now been shown to play a role in protecting mouse sperm from hydrogen peroxide toxicity (Zhang et al., 2008
International Patent WO2024003359A1 (2024)
10.1080/00498254.2023.2270046 246 ZangH.YangQ.LiJ
Therefore, the formation of IBs can pose an important obstacle during the production of recombinant proteins