Interestingly, GHK alone appeared to produce a comparable stimulatory action, while copper(II) ions in isolation may indicate no such activity, suggesting the tripeptide moiety may be the primary active component, possibly reforming complexes with trace copper present in the culture medium
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The microCT analysis was first used as a convenient method for histomorphometrical studies on long bones in ovariectomized rats and gene-deficient mice (50, 51)