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The supernatant was collected, adjusted to pH 4.5 using 2.5 M HCl and stored at 4 C for 12 h to facilitate protein precipitation

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Modulatory effects of BPC 157 on vasomotor tone and the activation of Src-Caveolin-1-endothelial nitric oxide synthase pathway Hsieh MJ, Lee CH, Chueh HY, Chang GJ, Huang HY, Lin Y, Pang JHS Scientific Reports (2020) This study demonstrates BPC-157's concentration-dependent vasodilation effect in isolated rat aorta through endothelium-dependent mechanisms