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Briefly, virgin Yolk-Gal4 or R4-Gal4 Drosophila were crossed with male w 1118 (control), UAS-CNCC-IR (dNrf2 knockdown), UAS-CNCC (dNrf2 overexpression), or UAS-Cyp18a1-IR (Cyp18a1 knockdown)

Contrasting low and high TMAO clusters revealed 215 differentially abundant bacterial species between these two groups (Fig