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To obtain monomeric fractions, SEC was performed on a Superdex 200 Increase 10/300 GL Column (Cytiva), as described above 97

This step is not only preparatory but also crucial in maintaining the sterility of the procedure, which helps prevent infection and ensures the treatment's effectiveness

However, direct cartilage injection of IGF-1 more effective than systemic elevation, and systemic growth hormone peptides produce modest IGF-1 increases (not pharmacological levels)

Cold-chain integrity must be evidenced

Those with high GIPR pathway activity may respond exceptionally well to tirzepatide or GLP-Squared

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