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Chromatin-immunoprecipitation coupled with quantitative PCR 48 hours post-transfection, confluent Drosophila S2R+ cells plated in 100 mm dishes were crosslinked with 1% formaldehyde and quenched for 5 minutes in 0.125 M Glycine as described in 21

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doi:10.1016/j.joca.2012.06.004 49

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[522] We calculated the cost of overhead, including fringe benefits, at 100 percent of the median hourly wage, consistent with previous years